Effect of Urtica dioica L extract on quantitative morphometric alterations of liver parenchymal cells in STZ diabetic rats

Diabetes is associated with several structural and functional liver abnormalities that affect glycogen and lipid metabolism. In this study, an attempt was made to evaluate the effects of hydroalcoholic extract of Urtica dioica leaves on Quantitative morphometric changes in parenchymal cells of the livers in STZ diabetic rats. Thirty male Wistar rats were allocated in 3 groups: normal, diabetic and treatment. Hyperglycemia was induced by 80 mg/kg Streptozotocin intraperitoneally. One week after the injection of STZ, the third group received the hydroalcoholic extract of Urtica dioica at 100 mg/kg/day over four weeks. After five weeks, the animals were sacrificed and whole livers were removed. Liver specimens were used for quantitative morphometric analyze after hematoxylin and eosin staining. All data are shown as means plus standard errors of means and were analyzed using One-Way ANOVA test at P<0.05.The mean area of hepatocytes, nuclei and nucleolus had a decrease in periportal zone and an increase in perivenous zone in the diabetic and treatment groups. The increase of hepatocyte area in perivenous zone and reduce of nucleus area in periportal zone was significant in the diabetic group in comparison with control group (P<0.05), but were not significant between treatment and diabetic group. This study showed that administration of 100 mg/kg/day of Urtica dioica leaves extracts after induction of diabetes can cause a little modulating in the main morphometric indices of liver such as area of hepatocytes, nuclei and nucleolus in periportal and perivenous zones

In vitro maturation of germinal vesicle oocytes in stimulated intracytoplasmic sperm injection cycles

Objective: This study evaluated in vitro maturation (IVM) of oocytes in the germinal vesicle (GV) stage in stimulated intracytoplasmic sperm injection (ICSI) cycles. Materials and Methods: A total of 26 women, aged 18-37 years, who were candidates for ICSI at the Fatemeh Zahra Infertility and Health Reproductive Research Center in 2007 were recruited for this study. We used the standard long protocol for ovarian stimulation. Follicles >11 mm were punctured 36-38 hours after administration of 10000 IU human chorionic gonadotrophin (hCG). Immature oocytes were cultured for 24-30 hours. Oocytes that liberated polar bodies were injected by sperm prepared within the previous day. IVM fertilized oocytes were cultured an additional 24-30 hours for cleavage. The rates of maturation, fertilization and cleavage in IVM oocytes were recorded and statistically compared to in vivo matured sibling oocytes. Results: There were 279 collected oocytes (mean±SD: 10.73 ±6.2), of which 4.08±2.79 were subjected to IVM. An average of 2.73 ±2.15 GV oocytes (70%) developed to metaphase II (MII). Although the maturation rate significantly differed between the IVM and in vivo Mil sibling oocyte groups (p=0.027), the numbers of fertilized oocytes (p=0.795) and cleaved embryos (p=0.529) were not significantly high in the in vivo group. Transfer of IVM embryos occurred in only three cases with one pregnancy that resulted in the delivery of a healthy baby. Conclusion: This study shows that culturing GV oocytes can produce acceptable numbers of four-cell embryos on the transfer day. The developmental competence of oocytes is not significantly different between early stage IVM and in vivo sibling embryos

Investigation on effects of ergosan^TM and vibromax^TM in control of white spot disease (WSD) and Vibriosis in shrimp Litopenaeus vannamei

Complementary feedstuff extract from Laminaria digitata and Ascophylum nodosum that containing 1% alginic acids as shrimps stimulating of immune system for control of WSSV is used in this study. The test shrimps Litopenaeus vannamei in larvae stage (Z1-PL1), post larvae stage (PL1-PL10) and juvenile (from 30th day to 40th day) fed by complimentary feedstuff comprised control group. The other condition in test and control group was the same manner. Both groups after 40 days exposed to WSSV by oral inoculation. The clinical sign and mortality recorded for 10 days. The results showed the survival rate of exposed group was higher than the control group and it was significant (p<0/05). The result also show the mortality observed in exposed group 48 hours later than the control group. This study can led us to new methods for control of White spot disease

Investigation the Impact of Exchange Rate Volatility on the Export of Agricultural Products

Introduction: Agricultural commodity export is a main attribute of developing countries and it is the basic force of development, however, developing countries have faced domestic and international instability in their markets and monetary and fiscal policies and these instabilities create a difficult condition for most of producers and exporters. Volatility in exchange market is one of the most important factor and vital concentrate for international trade especially agricultural commodity export. Because of this concern many studies have been conducted in this filed; (Aristotelous, 2001; Chen, 2009 and Sabuhoi and Piri, 2009) .Many of these studies has stated that exchange rate fluctuation has a negative impact on aggregated agricultural export; however, none of them has been focused on the effects of exchange rate fluctuation on exported value of important commodities in long run and short run. In recent years, exchange rate fluctuation has been raised about 6 percent since 2007 to 2010 and it seemed that this phenomenon has a negative impact on agricultural commodity export in Iran. To test this hypothesis exported value of three important commodity (date, orange and grape) and exchange rate volatility since 1970 to 2013 have been used. Material and Methods: In order to examine the relation between real exchange rate volatility and export values of date, orange and grapes, first GARCH method has been used to draw out exchange rate volatility; then, Panel unite root test has been used to check the level of integration. Since real exchange rate is not heterogeneous for different cross sections Levi-Lin and Chow unit root test has been used for this variable and IPS test has been applied to export value. Pederoni co-integration test has been used to check the integration between these variables. Finally, FMOLS (Fully Modified Ordinary Least Square) and DOLS (Dynamic Ordinary Least Square) methods have been used to estimate long run and short run coefficients. Results and Discussion: Main results showed that real exchange rate volatility and export value of selected commodities are Co-integrated. The coefficient estimation of FMOLS and DOLS methods are equal and statically significant; so, these methods aren’t statically different and they showed that real exchange rate volatility has a negative impact on exported value for whole panel. However, the specific coefficient for each commodity showed contradictory behavior in short run and long run; for example real exchange rate fluctuation has a negative and significant impact on all the commodities; but, in short run this variable has a positive and significant impact on exported value. Moreover, based on estimated results it seems that fluctuation in exchange market has a greater impact on more valuable commodities like date. Conclusion: Considering the importance of agricultural product trade and in order to overcome mono-product economy, this study investigated long term and short term relation between export of grape, orange, date and exchange rate volatilities. To this aim, first the index of exchange volatility using generated autoregressive conditional heteroscedasticity (GARCH) was calculated. In order to investigate the relation between exchange rate volatilities and export value of agricultural product, unit root test and cointegration test related to panel data were used during years 1971-2013. The results of model estimation showed that exchange rate volatilities in short term and long term have respectively positive and negative effects on the export value of orange, grape and date. In long term, the negative effects of Exchange rate volatilities on high-export-value products are more than its effects on low-export-value products. Based on the estimation results we can conclude that, in short run, exporters are willing to increase their interchange and gain profits of the volatility in exchange market; however, in long run exchange rate fluctuation has effect on their long run profit because of instability in price and production, especially for more valuable products. Moreover, these risks can be more harmful in long run because of its impact on investment and production and due to lack of production, most of Iranian exporters will lose their international markets, hence, their volume and values of agricultural commodities export will reduce gradually. Based on the results, Iranian government should create a stable economic condition in long run, present proper information about exchange market future trend and its fluctuation in long run and short run and create a better marketing condition in short run

HP0175 Promotes the Production of IL-23, IL-6, IL-1β and TGF-β

Helicobacter pylori infection induces a chronic gastric inflammatory infiltrate. This study was undertaken to evaluate the type of the innate immune responses elicited by the secreted peptidyl-prolyl cis-trans isomerase of H. pylori (HP0175). The cytokine production induced by HP0175 in neutrophils, and monocytes was evaluated. HP0175 was able to induce the expression of IL-23 in neutrophils, and monocytes, and IL-6, IL-1beta and TGF-beta in monocytes. These findings indicate that HP0175 is able to promote the activation of innate cells and the production of a cytokine milieu that may favour the development of Th17 response